New Acid Bath Technique Yekugadzira Yakachipa, Inotengeka Stem Cells
New Acid Bath Technique Yekugadzira Yakachipa, Inotengeka Stem Cells
Anonim

Kusvika parizvino, hunyanzvi huripo hwekugadziridza masero evakuru uye kuashandura kuita stem maseru aida nguva - angangoita mavhiki akati wandei - uye akave nekubudirira kwakashata kwechikamu chimwe muzana. Nhasi, boka revasayendisiti vekuJapan vakaburitsa tsvakiridzo yavo vachitsigira nzira iri nyore, inonzi stimulus-triggered acquisition of pluripotency (STAP), iyo inoshanda zvakanyanya, nekukurumidza, uye yakabudirira kupfuura nzira iripo. Muchokwadi, iyo nzira nyowani, yakaedzwa mukati memakonzo, inoda chete kuti masero eropa anyikwe muasidhi yekugezesa senzira yekuaburitsa kune yakaderera pH.

Pasinei nekuita kunge kureruka kwehunyanzvi uhwu hwekutanga, chirongwa chekusimudzira chakatora makore - angangoita mashanu - kubva Haruko Obokata, nyanzvi yezvinyorwa zvemasero emasero paRIKEN Center for Developmental Biology muKobe, Japan, akatanga kuuya nepfungwa yekutanga. Chimwe chikonzero kunonoka kwakareba kudaro kupararira pakati pekubata pamuviri nekuzvarwa inyaya yekuti aifanira kunyatsogutsa vaaishanda navo vesainzi nezvekukosha kwehunyanzvi hwake. Asi iyo, sezvavanotaura, isayenzi.

Masero maseru maseru asina hunyanzvi ane kugona kunoshamisa kushanduka kuita chero sero mumuviri wemunhu uye nechikonzero ichi inzvimbo yakakosha yekutsvagisa kune masayendisiti ari kutsvaga kugadzira mushonga wemunhu. Iyo tekinoroji yemajini ekugadziridza maseru uye kugadzira stem masero, anozivikanwa se induced pluripotent stem masero (iPSC), yakatanga kutaurwa muna 2006 uye kuratidzwa mumasero emunhu gore rakatevera. Kubva ipapo, iPSCs yakave yakakosha kune masayendisiti mukuvandudza zvinodhaka uye kuenzanisira kwezvirwere, kunyangwe maitiro ekugadzira aya maseru, ayo aitora uye aisawanzo budirira, akasiya zvakanyanya kudiwa.

Saka, nzira itsva ye STAP cell yakauya sei? Rimwe zuva achigadzira masero, Obokata akaona kuti mamwe masero, mushure mekudzvanywa kuburikidza necapillary chubhu, akaonda kusvika pakukura kwakafanana semasero. Akazvibvunza kuti: Kungosimbisa maseru kunogona kuvaita pluripotent here? Kuti aongorore, akatanga nhevedzano yekuyedza kwaakashandisa akasiyana marudzi ekushushikana, kusanganisira kupisa, nzara, uye yakakwirira-calcium nharaunda. Nenguva isipi, akaona kuti zvinonetsa zvitatu - perforating cell membrane ine muchetura webhakitiriya, kudzvanya kwemuviri, uye kufumura sero kune yakaderera pH - yakakonzera zviratidzo zvepluripotency mumaseru.

Mushure mekuita kuwanikwa uku kwakakosha, Obokata akatevera kuratidza kuti masero aya aikwanisa kushanduka kuita chero uye ese marudzi esero nenzira yechokwadi stem cell. Kuti aite izvi, akatendeukira kuna piyona wembeva Teruhiko Wakayama paYunivhesiti yeYamanashi, Japan, uyo akamubatsira nekugadzira mbeva dzaaigona kupinza masero ake eSTAP. Zvadaro, akashanda murabhoritari yaCharles Vacanti, injiniya wetishu paHarvard University. Nerubatsiro rwake, Obokata akafumura tsananguro yebhaibheri yenzira yake: chizvarwa ichocho chemasero nenzira iyi chinoitika mumasikirwo semhinduro yekukuvara.

Nhasi, Obokata yakatove yakagadziridza masero gumi nemaviri emarudzi, kusanganisira ayo anobva kunhengo dzakasiyana dzemuviri. Kunyange zvazvo achitenda kuti STAP nzira haishande pamhando dzose dzemasero, inoshanda nevakawanda uye, paavhareji, 25 muzana yemasero inopona kushushikana uye 30 muzana yeavo vanotendeukira kumasero epluripotent. Tsvagiridzo yake inoonekwa mumapepa maviri akaburitswa panguva imwe chete muNature, pamwe nepfupiso yemupepeti ichiti, "Kuongorora kwakadzama kwemamorekuru uye kugona kwekusimudzira kwemasero eSTAP kunoratidza kuti anomiririra yakasarudzika mamiriro epluripotency - uye anopa imwe sosi yemaseru epluripotent …”

Kwakabva

Obokata H, Wakayama T, Sasai Y, Kojima K, Vacanti MP, et al. Stimulus-yakakonzeresa fate shanduko ye somatic masero kuita pluripotency. Nature. 2014.

Obokata H, Wakayama T, Sasai Y, Niwa H, Kadota M, Takata N, et al. Bidirectional budiriro inogoneka mumasero akagadziridzwa ane akawana pluripotency. Nature. 2014.

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