Vatsvakurudzi vanowana nzira yakanakisisa yekuona utachiona hwemhepo
Vatsvakurudzi vanowana nzira yakanakisisa yekuona utachiona hwemhepo
Anonim

Nzira dzazvino dzinoshandiswa kufemba utachiona hune ngozi hwemhepo dzingakarakadza kuti hapana tyisidziro kuutano apo, chaizvoizvo, ingavapo.

Asi vatsvakurudzi vakawana nzira iri nani yekuunganidza nekuongorora utachiona uhu hunogona kupa ongororo yechokwadi yekutyisidzira kwavo chaiko. Semuenzaniso, zvakawanikwa zvinogona kuita kuti zvive nyore kuona hutachiona hwemhepo munzvimbo dzakaderera.

"Zvatinowana zvinoratidza kuti nzira dzesampling dzinowanzo shandiswa dzinongoona chikamu chidiki chezviri mumhepo," akadaro Timothy Buckley, munyori mukuru wechidzidzo ichi uye purofesa wezvehutano hweveruzhinji kuOhio State.

"Uye izvo zvavanoona zvinowanzokuvadzwa - nekuda kwenzira yekuunganidza - zvekuti hutachiona hahuchina hutachiwana hwakafanana nehwavaive vari mumhepo."

Kukuvadzwa kwakadaro kunogona kuita kuti zvisaite kuti vashandi vehutano veveruzhinji vaone kana pathogen ichigoneka - kureva kuti, ingave ine mukana wekutapurira kana kwete.

Buckley nevamwe vake vakawana kuti mudziyo mutsva unonzi BioSampler wakakonzera kukuvadzwa kushoma kune tupukanana tusina kutapurira hunoshandiswa muchidzidzo ichi. Iyo BioSampler yakagadzirwa mukupera kwema1990 nechikwata chevaongorori kubva kuYunivhesiti yeCincinnati. Kunyangwe isati iri nzira inowanzoshandiswa yekuona utachiona hwemhepo, zvakapa Buckley nechikwata chake kuverenga kwakanyatsojeka kwedhigirii rekuita kwe microorganism, kugona kwayo kukura mumuviri wemunhu.

Mhedzisiro parizvino inoonekwa online pawebhusaiti yejenari Environmental Science & Technology. Ana Rule, muongorori wepashure paJohns Hopkins University Bloomberg School of Public Health, akatungamira chidzidzo ichi.

Mukutevedzana kwezviyedzo, vaongorori vakaedza BioSampler pamwe chete nemaitiro maviri echinyakare anoshandiswa kuyedza mhepo - iri nyore membrane sefa, inoteya tupukanana pane yakarukwa-yakasimba mesh skrini, uye iyo AGI-30 (All-Glass Impinger-30), iyo inounganidza zvipenyu mukamuri yegirazi izere nemvura. Nepo iyo BioSampler inounganidzawo tupukanana mukamuri yegirazi, dhizaini yayo yakati siyanei. Musiyano iwoyo unogona kukonzera kukuvara kushoma sezvo chipenyu chakavharirwa, Buckley akadaro.

Vatsvakurudzi vakashandisa Pantoea agglomerans, bhakitiriya risina pathogenic iyo ihama iri kure neE. coli uye kuYersenia pestis, bhakitiriya inokonzera denda. Y. pestis iri pakati pezvirwere zvakanyorwa neCenters for Disease Control and Prevention seCategory A bioterrorism agent, zvichireva kuti inotapuriranwa nyore nyore kubva kumunhu kuenda kumunhu uye inogona kukonzera huwandu hwevanhu vanofa. E. coli inogona kusvibisa chikafu nemvura uye zvakare kuisa njodzi yemhepo, Buckley akadaro.

Vatsvakurudzi vakaisa sampuli yeP. agglomerans pamudziyo wega wega kuti vaone kuti mudziyo uyu wainyatsogona sei kuchengetedza microorganism payakatanga uye kuti ndeipi nzira yesampling yakakuvadza P. agglomerans zvishoma. Vakaunganidza sampuli dzehutachiona kubva kune yega yega mudziyo mushure menguva yakafanotemerwa, uye ndokuyedza kugona kwehutachiona - ndiko kuti, kana kwete yemuviri cellular membrane yaive yakasimba. Vakaedzawo kukura sample imwe neimwe mundiro yemurabhoritari, hunhu hunonzi nevanoongorora pathogen's "culturability," iyo inoratidza kuti chipenyu chacho chinogona kutevedzera uye kukura here.

"Nezvehutano, chiyero chegoridhe chazvino ndechekuona kana pathogen inogona kukura murabhoritari," Rule akadaro. "Muchokwadi, kugona kwayo kunogona kunge kuri kuongorora kwakaringana kwekugona kwayo kutyisidzira hutano hwevanhu.

"Kunyangwe payakakuvadzwa nesampling process, hutachiona hune nzira dzekugadzirisa izvo nekufamba kwenguva mune chaiyo svikiro - semuenzaniso, muviri wemunhu - unovabvumira kutevedzera uye kukura," akaenderera mberi. "Kukura maseru mukati mekukurumidza mushure mesampling kunogona kusanyatsomiririra zvinoitika biologically."

Vatsvakurudzi vakashandisa nzira inonzi flow cytometry kuongorora kushanda kweP. agglomerans zvipenyu zvakaunganidzwa nenzira yega yega yesampling. Mukuyerera kwe cytometry, masero akasvibiswa nedhayi yefluorescent uye anopfuura nepakati pechiedza chelaser light. Ruvara rwunobuda, rwunoonekwa nevesainzi sezvo zvipenyu zvichipfuura nechiedza ichi, hunovaudza kuti masero mupenyu (anoshanda) kana akafa.

"Nekuda kwekuti isu hatigone kurima chimwe chinhu chiri pakati nepakati murabhoritari hazvireve kuti hachizokura mumunhu," Buckley akadaro.

Flow cytometry inoudzawo vaongorori kuti mangani maseru akazara mune yakapihwa sampuli, izvo zvinovatendera kuti vaone kana chero maseru akarasika nenzira yesampling (vakange vaona maverengero chaiwo mumuenzaniso wega wega webhakitiriya vasati vashandisa nzira yega yega.)

Kune yega yega sampling nzira, vaongorori vakaongorora huwandu hwese, hunoshanda uye huchikura mabhakitiriya.

Vakaona kuti maseru akapetwa katatu kana katanhatu aigoneka kupfuura kugadzirwa mushure mekushandisa nzira yekusefa uye AGI-30, zvichiratidza kuti mazhinji eP. agglomerans akange akuvadzwa mukuunganidza. Asi P. agglomerans samples dzakatorwa kubva kuBioSampler dzakaratidza chibvumirano chepedyo pakati pehuwandu hwehutachiona hunoshanda uye hunokura. Yakanga iriwo sampler ine mashoma ekurasikirwa kwesero.

"Kubva pane izvi zvabuda, zvakanaka kugumisa kuti idzo nzira dzekuongorora dzakajairika dzinogona kusagara dzichimiririra kuvepo chaiko uye kuumbwa kwehutachiona," Buckley akadaro. "Kunzwisisa zvinokonzeresa nzira yesampling pane pathogen kwakakosha pakugadzira nzira dziri nani dzesampling."

Buckley uye Rule vakaita basa naKellogg Schwab, purofesa anobatana neJohns Hopkins School of Public Health, naJana Kesavan, ane Aerosol Sciences Team, RDECOM, paEdgewood Chemical uye Biological Center muEdgewood, Md.

Mari yebasa iri yakapihwa neU.S. Army Edgewood Chemical and Biological Center kuburikidza neScientific Services Agreement neBattelle.

Chidzidzo ichi chikamu cheOhio States itsva Targeted Investment in Excellence (TIE) chirongwa mukugadzirira hutano hweveruzhinji. Chirongwa cheTIE chakanangana nedzimwe nharaunda dzinonetsa zvakanyanya nekudyara kukuru kwezviwanikwa zveyunivhesiti muzvirongwa zvine mukana wekuita zvakakosha muminda yavo. Yunivhesiti yakazvipira kudarika $100 miriyoni mumakore mashanu anotevera kutsigira 10 yakakwirira-impact, kazhinji zvirongwa zvepakati pedisciplinary.

Yakakurumbira nenyaya