Nanoblasts kubva laser-activated nanoparticles inofambisa mamorekuru, mapuroteni uye DNA mumasero
Nanoblasts kubva laser-activated nanoparticles inofambisa mamorekuru, mapuroteni uye DNA mumasero
Anonim

Vachishandisa kemikari "nanoblasts" inobaya maburi madiki mumaseru anodzivirira, vaongorori vakaratidza nzira nyowani yekuwana kurapa mamorekuru madiki, mapuroteni uye DNA zvakananga mumasero mapenyu.

Carbon nanoparticles inobatiswa nekuputika kwelaser light inokonzeresa kuputika kudiki, kunovhura maburi mumasero membranes akareba kuti abvumire mishonga yekurapa iri mumvura yakatenderedza. Nekugadzirisa laser exposure, vaongorori vakapa diki-morekuru mamaki komisheni kusvika makumi mapfumbamwe muzana emaseru anonangwa - vachichengeta anopfuura makumi mapfumbamwe muzana emaseru ari mupenyu.

Tsvagiridzo iyi yakatsigirwa neNational Institutes of Health uye Institute of Paper Science uye Technology kuGeorgia Tech. Ichashumwa munyaya yaAugust yemagazini Nature Nanotechnology.

"Iyi nzira inogona kutibvumira kuendesa zvakasiyana-siyana zvekurapa izvo zvino zvisingakwanisi kupinda mumasero," akadaro Mark Prausnitz, purofesa muChikoro cheChemical uye Biomolecular Engineering kuGeorgia Institute of Technology. "Chimwe chekushandiswa kwakakosha kweiyi tekinoroji inogona kunge iri yegene-based therapies, iyo inopa chivimbiso chikuru mukurapa, asi iyo kufambira mberi kwayo kwakaganhurirwa nekuoma kwekuwana DNA neRNA mumasero."

Iro basa rinofungidzirwa kuve rekutanga kushandisa activation ye reactive kabhoni nanoparticles nema lasers ekurapa maapplication. Kuwedzera kutsvagisa uye kuyedzwa kwekiriniki kunozodiwa nzira iyi isati yashandiswa muvanhu.

Vatsvagiri vanga vachiedza kwemakumi emakore kutyaira DNA neRNA zvakanyanya mumaseru nenzira dzakasiyana siyana, kusanganisira kushandisa mavhairasi kutakura genetic zvinhu mumaseru, kuvhara DNA neRNA nemakemikari ejenti kana kushandisa minda yemagetsi uye ultrasound kuvhura maseru membranes. Nekudaro, idzi nzira dzakapfuura dzakawanzo tambura nekushomeka kwekuita kana kuchengetedzeka kunetseka.

Nemaitiro avo matsva, ayo akafemerwa nebasa rekare pane inonzi "photoacoustic effect," Prausnitz nevabatsiri Prerona Chakravarty, Wei Qian naMostafa El-Sayed vanotarisira kuisa zvirinani kushandiswa kwesimba kune membranes yesero, kugadzira yakachengeteka uye yakachengeteka. nzira inoshanda kwazvo yekuendesa zvinodhaka intracellular.

Maitiro avo anotanga nekuunza zvimedu zvekabhoni nhema inoyera 25 nanometers - miriyoni imwe yemainji - mudhayamita mumvura yakatenderedza maseru umo mishonga yekurapa ichapinzwa. Kuputika kwechiedza chepedyo-infrared kubva kufemotosecond laser kunozoiswa kumvura pamwero we90 miriyoni pulses pasekondi. Iyo kabhoni nanoparticles inotora mwenje, izvo zvinoita kuti dzipise. Zvimedu zvinopisa zvinobva zvadziisa mvura yakapoteredza kuita chipfunga. Iyo steam inopindirana nekabhoni nanoparticles kuumba hydrogen uye carbon monoxide.

Iwo magasi maviri anoumba bubble iro rinokura sezvo laser inopa simba. Iyo bubble inodonha kamwe kamwe kana laser yakadzimwa, ichigadzira kuvhunduka kunobaya maburi mumembrane yemaseru ari pedyo. Iwo mazaru anobvumira mishonga yekurapa kubva kune yakatenderedza fluid kupinda mumasero. Makomba anovhara nekukurumidza kuti sero rirarame.

Vatsvakurudzi vakaratidza kuti vaigona kuwana iyo diki molecule calcein, bovine serum albumin protein uye plasmid DNA kuburikidza nesero membranes emunhu masero egomarara reprostate uye makonzo gliosarcoma masero vachishandisa nzira iyi. Calcein kutora kwakaonekwa mu90 muzana yemaseru pamazinga e laser akasiya anopfuura makumi mapfumbamwe muzana emasero ari mupenyu.

"Tinogona kuwana masero ese kuti atore mamorekuru aya aisazopinda mumaseru, uye anenge maseru ese akaramba ari mupenyu," akadaro Prerona Chakravarty, munyori anotungamira wechidzidzo ichi. "Yedu laser-activated kabhoni nanoparticle system inogonesa inodzorwa bubble implosions inogona kukanganisa masero membranes zvakakwana kuti apinze mamorekuru mukati pasina kukanganisa kusingaperi."

Kuti utarise kuti maburi ari musero membrane akasara akavhurika kwenguva yakareba sei, vaongorori vakasiya marapirwo akateedzerwa kunze kwemvura apo masero akaiswa kune mwenje welaser, ndokuwedzera maagent sekondi imwe mushure mekudzima laser. Vakaona kunenge kusisatore mamorekuru, zvichiratidza kuti maseru anozvivharisa nekukurumidza.

Kusimbisa kuti kabhoni-steam reaction yaive yakakosha kutyaira nanoblasts, vaongorori vakatsiva goridhe nanoparticles kune kabhoni nanoparticles vasati vasangana nelaser mwenje. Nekuda kwekuti vaishaya kabhoni yaidiwa kuita, nanoparticles yegoridhe yakaburitsa zvishoma zvekutora mamorekuru, Prausnitz akadaro.

Saizvozvo, vaongorori vakatsiva kabhoni nanotubes kune kabhoni nanoparticles, uye zvakare akayera kukwira kushoma, izvo zvavakatsanangura nekuona kuti nanotubes haina kuita zvishoma pane kabhoni dema particles.

Kuedza kwakaratidza zvakare kuti DNA yakaunzwa mumaseru kuburikidza nelaser-activated tekiniki yakaramba ichishanda uye ichikwanisa kutyaira mapuroteni kutaura. Pakaunzwa plasmid DNA iyo yakavharirwa kutaura kwe luciferase mumasero egomarara, kugadzirwa kwe luciferase kwakawedzera kagumi nenomwe.

Kune ramangwana, vaongorori vanoronga kudzidza kushandisa isingadhure nanosecond laser kutsiva iyo yekupedzisira femtosecond chiridzwa chinoshandiswa mukutsvaga. Ivo zvakare vanoronga kukwidziridza iyo kabhoni nanoparticles kuitira kuti dzinenge dzese dzipere panguva yekuratidzwa kwelaser mwenje. Yakasara kabhoni nanoparticles mumuviri haifanirwe kuburitsa zvinokuvadza, kunyangwe muviri ungatadza kuzvibvisa, Prausnitz akadaro.

"Ichi chidzidzo chekutanga chinoratidza humbowo hwemusimboti welaser-activation ye reactive kabhoni nanoparticles yezvinodhaka uye gene kuendesa," akadaro. "Pane nzira yakakura pamberi apa isati yaunzwa mukurapa, asi isu tine tarisiro yekuti nzira iyi inogona kupedzisira yapa imwe nzira yekuunza varapi mumaseru zvisina njodzi uye nemazvo."

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